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anti glun1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti glun1
    Anti Glun1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+glun1/AMPA+Receptor+2+(GluA2)+Rabbit+mAb/pm41700129-55-46-47
    Average 95 stars, based on 88 article reviews
    anti glun1 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Increased NMDARs in neurons and glutamine synthetase in astrocytes underlying autistic-like behaviors of Gabrb1 −/− mice
    Article Snippet: Then the mixture was incubated with Protein A/G Plus-Agarose (SC-2003, Santa Cruz, USA) for 3 hours at 4°C. .. After centrifugation at 1,000 g for 5 min at 4°C, the immunoprecipitants were washed four times with lysis buffer and then boiled in protein loading buffer for 5 min. Western blotting was performed to detect the proteins using the following primary antibodies: rabbit anti-SHANK3 (64555S, Cell Signaling Technology, USA; 1:1,000), rabbit anti-GluN1 (5704S, Cell Signaling Technology, USA; 1:1,000), rabbit anti-GluN2B (14544S, Cell Signaling Technology, USA; 1:1,000), rabbit anti-GABRB1 (20183-1-AP, Proteintech, USA; 1:800), mouse anti-GAPDH (60004-1-Ig, Proteintech, USA; 1:10,000), mouse anti-PSD95 (ab2723, Abcam, UK; 1:1,200), rabbit anti-Synaptophysin (ab52636, Abcam, UK; 1:4,000). ..

    Lysis:

    Article Title: Increased NMDARs in neurons and glutamine synthetase in astrocytes underlying autistic-like behaviors of Gabrb1 −/− mice
    Article Snippet: Then the mixture was incubated with Protein A/G Plus-Agarose (SC-2003, Santa Cruz, USA) for 3 hours at 4°C. .. After centrifugation at 1,000 g for 5 min at 4°C, the immunoprecipitants were washed four times with lysis buffer and then boiled in protein loading buffer for 5 min. Western blotting was performed to detect the proteins using the following primary antibodies: rabbit anti-SHANK3 (64555S, Cell Signaling Technology, USA; 1:1,000), rabbit anti-GluN1 (5704S, Cell Signaling Technology, USA; 1:1,000), rabbit anti-GluN2B (14544S, Cell Signaling Technology, USA; 1:1,000), rabbit anti-GABRB1 (20183-1-AP, Proteintech, USA; 1:800), mouse anti-GAPDH (60004-1-Ig, Proteintech, USA; 1:10,000), mouse anti-PSD95 (ab2723, Abcam, UK; 1:1,200), rabbit anti-Synaptophysin (ab52636, Abcam, UK; 1:4,000). ..

    Western Blot:

    Article Title: Increased NMDARs in neurons and glutamine synthetase in astrocytes underlying autistic-like behaviors of Gabrb1 −/− mice
    Article Snippet: Then the mixture was incubated with Protein A/G Plus-Agarose (SC-2003, Santa Cruz, USA) for 3 hours at 4°C. .. After centrifugation at 1,000 g for 5 min at 4°C, the immunoprecipitants were washed four times with lysis buffer and then boiled in protein loading buffer for 5 min. Western blotting was performed to detect the proteins using the following primary antibodies: rabbit anti-SHANK3 (64555S, Cell Signaling Technology, USA; 1:1,000), rabbit anti-GluN1 (5704S, Cell Signaling Technology, USA; 1:1,000), rabbit anti-GluN2B (14544S, Cell Signaling Technology, USA; 1:1,000), rabbit anti-GABRB1 (20183-1-AP, Proteintech, USA; 1:800), mouse anti-GAPDH (60004-1-Ig, Proteintech, USA; 1:10,000), mouse anti-PSD95 (ab2723, Abcam, UK; 1:1,200), rabbit anti-Synaptophysin (ab52636, Abcam, UK; 1:4,000). ..

    Article Title: Mdga2 deficiency leads to an aberrant activation of BDNF/TrkB signaling that underlies autism-relevant synaptic and behavioral changes in mice.
    Article Snippet: Protease (Cat# 4693132001) and phosphatase (Cat# 4906845001) inhibitor cocktails were purchased from Roche. .. Primary antibodies used include: rabbit anti-β-actin (Cell Signaling Technology, Cat# 8457); rabbit anti-Akt (Cell Signaling Technology, Cat# 4685); rabbit anti-pAkt (Cell Signaling Technology, Cat# 4060); rabbit anti-BDNF (Abcam, Cat# ab108319); rabbit anti-CaMKII (Cell Signaling Technology, Cat# 3357S); rabbit anti-pCaMKII (Cell Signaling Technology, Cat# PLOS Biology | https://doi.org/10.1371/journal.pbio.3003047 April 1, 2025 19 / 33 12716); rabbit anti-Flag (Proteintech, Cat# 20543-1-AP); rabbit anti-GAD1 (Proteintech, Cat# 10408-1-AP); mouse anti-Gephyrin (Synaptic Systems, Cat# 147 011); rabbit anti-Gephyrin (Proteintech, Cat# 12681-1-AP); mouse anti-GFAP (Cell Signaling Technology, Cat# 3670); mouse anti-GFP (Abmart, Cat# M20004); rabbit anti-Iba1 (Cell Signaling Technology, Cat# 17198); rabbit anti-MDGA1 (Biorbyt, Cat# orb773819); rabbit anti-MDGA2 (Abcam, Cat# ab121164, for immunostaining); rabbit anti-MDGA2 (Biorbyt, Cat# orb540377, for immunoblotting); rabbit anti-mTOR (Cell Signaling Technology, Cat# 2983); rabbit anti-pmTOR (Cell Signaling Technology, Cat# 5536); mouse anti-NeuN (Cell Signaling Technology, Cat# 94403); rabbit anti-NLGN1 (Thermo Fisher Scientific, Cat# PA5-78648); mouse anti-phosphotyrosine (Millipore, Cat# 16-316); rabbit anti-PSD-93 (Abcam, Cat# ab151721); mouse anti-PSD-95 (Synaptic Systems, Cat# 124 011); rabbit anti-PSD-95 (Cell Signaling Technology, Cat# 3450S); mouse anti-puromycin antibody (Millipore, Cat# MABE343); rabbit anti-S6 (Cell Signaling Technology, Cat# 2217); rabbit anti-pS6 (Cell Signaling Technology, Cat# 2211); mouse anti-Synaptophysin (Sigma-Aldrich, Cat# S5768); rabbit anti-TrkB (Cell Signaling Technology, Cat# 4603); rabbit anti-pTrkB (Cell Signaling Technology, Cat# 4619); rabbit anti-GluA1 (Cell Signaling Technology, Cat# 13185); rabbit anti-GluN1 (Cell Signaling Technology, Cat# 5704); mouse anti-vGAT (Santa Cruz Biotechnology, Cat# sc-393373); and mouse antivGluT1 (Millipore, Cat# MAB5502). .. The secondary antibodies used include: Goat anti-Mouse IgG (H + L), HRP (ThermoFisher, Cat# 31430); Goat anti-Rabbit IgG (H + L), HRP (ThermoFisher, Cat# 31460); Alexa Fluor 635 Goat anti-Mouse IgG (H + L) (ThermoFisher, Cat# A-31575); Alexa Fluor 488 Goat anti-Rabbit IgG (H + L) (ThermoFisher, Cat#A-11008); and Alexa Fluor 594 Goat anti-Rat IgG (H + L) (ThermoFisher, Cat# A-11007).

    Article Title: Mdga2 deficiency leads to an aberrant activation of BDNF/TrkB signaling that underlies autism-relevant synaptic and behavioral changes in mice
    Article Snippet: Protease (Cat# 4693132001) and phosphatase (Cat# 4906845001) inhibitor cocktails were purchased from Roche. .. Primary antibodies used include: rabbit anti-β-actin (Cell Signaling Technology, Cat# 8457); rabbit anti-Akt (Cell Signaling Technology, Cat# 4685); rabbit anti-pAkt (Cell Signaling Technology, Cat# 4060); rabbit anti-BDNF (Abcam, Cat# ab108319); rabbit anti-CaMKII (Cell Signaling Technology, Cat# 3357S); rabbit anti-pCaMKII (Cell Signaling Technology, Cat# 12716); rabbit anti-Flag (Proteintech, Cat# 20543-1-AP); rabbit anti-GAD1 (Proteintech, Cat# 10408-1-AP); mouse anti-Gephyrin (Synaptic Systems, Cat# 147 011); rabbit anti-Gephyrin (Proteintech, Cat# 12681-1-AP); mouse anti-GFAP (Cell Signaling Technology, Cat# 3670); mouse anti-GFP (Abmart, Cat# M20004); rabbit anti-Iba1 (Cell Signaling Technology, Cat# 17198); rabbit anti-MDGA1 (Biorbyt, Cat# orb773819); rabbit anti-MDGA2 (Abcam, Cat# ab121164, for immunostaining); rabbit anti-MDGA2 (Biorbyt, Cat# orb540377, for immunoblotting); rabbit anti-mTOR (Cell Signaling Technology, Cat# 2983); rabbit anti-pmTOR (Cell Signaling Technology, Cat# 5536); mouse anti-NeuN (Cell Signaling Technology, Cat# 94403); rabbit anti-NLGN1 (Thermo Fisher Scientific, Cat# PA5-78648); mouse anti-phosphotyrosine (Millipore, Cat# 16-316); rabbit anti-PSD-93 (Abcam, Cat# ab151721); mouse anti-PSD-95 (Synaptic Systems, Cat# 124 011); rabbit anti-PSD-95 (Cell Signaling Technology, Cat# 3450S); mouse anti-puromycin antibody (Millipore, Cat# MABE343); rabbit anti-S6 (Cell Signaling Technology, Cat# 2217); rabbit anti-pS6 (Cell Signaling Technology, Cat# 2211); mouse anti-Synaptophysin (Sigma-Aldrich, Cat# S5768); rabbit anti-TrkB (Cell Signaling Technology, Cat# 4603); rabbit anti-pTrkB (Cell Signaling Technology, Cat# 4619); rabbit anti-GluA1 (Cell Signaling Technology, Cat# 13185); rabbit anti-GluN1 (Cell Signaling Technology, Cat# 5704); mouse anti-vGAT (Santa Cruz Biotechnology, Cat# sc-393373); and mouse anti-vGluT1 (Millipore, Cat# MAB5502). .. The secondary antibodies used include: Goat anti-Mouse IgG (H + L), HRP (ThermoFisher, Cat# 31430); Goat anti-Rabbit IgG (H + L), HRP (ThermoFisher, Cat# 31460); Alexa Fluor 635 Goat anti-Mouse IgG (H + L) (ThermoFisher, Cat# A-31575); Alexa Fluor 488 Goat anti-Rabbit IgG (H + L) (ThermoFisher, Cat#A-11008); and Alexa Fluor 594 Goat anti-Rat IgG (H + L) (ThermoFisher, Cat# A-11007).

    other:

    Article Title: Increased NMDARs in neurons and glutamine synthetase in astrocytes underlying autistic-like behaviors of Gabrb1 −/− mice
    Article Snippet: rabbit anti-GluN1 , Cell Signaling Technology , Cat. # 5704S; RRID: AB_1904067.

    Incubation:

    Article Title: Phosphorylated Ser187-SNAP25-modulated hyperfunction of glutamatergic system in the vmPFC mediates depressive-like behaviors in male mice.
    Article Snippet: Dysfunctional glutamatergic neurotransmission contributes importantly to the pathophysiology of depression.. However, the underlying neural mechanisms of glutamatergic dysfunction remain poorly understood.. Here, we employed chronic unpredictable mild stress (CUMS) to induce depression-like behavior in male mice and to assess the alterations of glutamatergic system within the ventromedial prefrontal cortex (vmPFC).

    Article Title: Dopamine D1 and NMDA Receptor Co-Regulation of Protein Translation in Cultured Nucleus Accumbens Neurons.
    Article Snippet: .. Cells were then incubated with one of the following primary antibodies overnight at 4 °C: 1:2000 rat anti-D1R (D2944, Sigma Aldrich; gift of Dr. Susan George, University of Toronto), 1:1000 rabbit anti-GluN1 (D65B7 #5704S; Cell Signaling Technology), or 1:1000 mouse antityrosine hydroxylase (#22941; Immunostar, Hudson, WI). .. Coverslips were then washed with PBS and incubated in 1:500 Cy3-conjugated rabbit anti-mouse (PA43009V, GE Healthcare, Buckinghamshire, UK), 1:500 Cy3-conjugated goat anti-rabbit (28901106 V, GE Healthcare) or goat antirat (A10525, Invitrogen, Carlsbad, CA) secondary antibody for 1 h at room temperature before being mounted on slides using ProLong Gold Antifade mountant (Thermo Fisher, Waltham, MA).

    Gentle:

    Article Title: Phosphorylated Ser187-SNAP25-modulated hyperfunction of glutamatergic system in the vmPFC mediates depressive-like behaviors in male mice.
    Article Snippet: Dysfunctional glutamatergic neurotransmission contributes importantly to the pathophysiology of depression.. However, the underlying neural mechanisms of glutamatergic dysfunction remain poorly understood.. Here, we employed chronic unpredictable mild stress (CUMS) to induce depression-like behavior in male mice and to assess the alterations of glutamatergic system within the ventromedial prefrontal cortex (vmPFC).

    Immunostaining:

    Article Title: Mdga2 deficiency leads to an aberrant activation of BDNF/TrkB signaling that underlies autism-relevant synaptic and behavioral changes in mice.
    Article Snippet: Protease (Cat# 4693132001) and phosphatase (Cat# 4906845001) inhibitor cocktails were purchased from Roche. .. Primary antibodies used include: rabbit anti-β-actin (Cell Signaling Technology, Cat# 8457); rabbit anti-Akt (Cell Signaling Technology, Cat# 4685); rabbit anti-pAkt (Cell Signaling Technology, Cat# 4060); rabbit anti-BDNF (Abcam, Cat# ab108319); rabbit anti-CaMKII (Cell Signaling Technology, Cat# 3357S); rabbit anti-pCaMKII (Cell Signaling Technology, Cat# PLOS Biology | https://doi.org/10.1371/journal.pbio.3003047 April 1, 2025 19 / 33 12716); rabbit anti-Flag (Proteintech, Cat# 20543-1-AP); rabbit anti-GAD1 (Proteintech, Cat# 10408-1-AP); mouse anti-Gephyrin (Synaptic Systems, Cat# 147 011); rabbit anti-Gephyrin (Proteintech, Cat# 12681-1-AP); mouse anti-GFAP (Cell Signaling Technology, Cat# 3670); mouse anti-GFP (Abmart, Cat# M20004); rabbit anti-Iba1 (Cell Signaling Technology, Cat# 17198); rabbit anti-MDGA1 (Biorbyt, Cat# orb773819); rabbit anti-MDGA2 (Abcam, Cat# ab121164, for immunostaining); rabbit anti-MDGA2 (Biorbyt, Cat# orb540377, for immunoblotting); rabbit anti-mTOR (Cell Signaling Technology, Cat# 2983); rabbit anti-pmTOR (Cell Signaling Technology, Cat# 5536); mouse anti-NeuN (Cell Signaling Technology, Cat# 94403); rabbit anti-NLGN1 (Thermo Fisher Scientific, Cat# PA5-78648); mouse anti-phosphotyrosine (Millipore, Cat# 16-316); rabbit anti-PSD-93 (Abcam, Cat# ab151721); mouse anti-PSD-95 (Synaptic Systems, Cat# 124 011); rabbit anti-PSD-95 (Cell Signaling Technology, Cat# 3450S); mouse anti-puromycin antibody (Millipore, Cat# MABE343); rabbit anti-S6 (Cell Signaling Technology, Cat# 2217); rabbit anti-pS6 (Cell Signaling Technology, Cat# 2211); mouse anti-Synaptophysin (Sigma-Aldrich, Cat# S5768); rabbit anti-TrkB (Cell Signaling Technology, Cat# 4603); rabbit anti-pTrkB (Cell Signaling Technology, Cat# 4619); rabbit anti-GluA1 (Cell Signaling Technology, Cat# 13185); rabbit anti-GluN1 (Cell Signaling Technology, Cat# 5704); mouse anti-vGAT (Santa Cruz Biotechnology, Cat# sc-393373); and mouse antivGluT1 (Millipore, Cat# MAB5502). .. The secondary antibodies used include: Goat anti-Mouse IgG (H + L), HRP (ThermoFisher, Cat# 31430); Goat anti-Rabbit IgG (H + L), HRP (ThermoFisher, Cat# 31460); Alexa Fluor 635 Goat anti-Mouse IgG (H + L) (ThermoFisher, Cat# A-31575); Alexa Fluor 488 Goat anti-Rabbit IgG (H + L) (ThermoFisher, Cat#A-11008); and Alexa Fluor 594 Goat anti-Rat IgG (H + L) (ThermoFisher, Cat# A-11007).

    Article Title: Mdga2 deficiency leads to an aberrant activation of BDNF/TrkB signaling that underlies autism-relevant synaptic and behavioral changes in mice
    Article Snippet: Protease (Cat# 4693132001) and phosphatase (Cat# 4906845001) inhibitor cocktails were purchased from Roche. .. Primary antibodies used include: rabbit anti-β-actin (Cell Signaling Technology, Cat# 8457); rabbit anti-Akt (Cell Signaling Technology, Cat# 4685); rabbit anti-pAkt (Cell Signaling Technology, Cat# 4060); rabbit anti-BDNF (Abcam, Cat# ab108319); rabbit anti-CaMKII (Cell Signaling Technology, Cat# 3357S); rabbit anti-pCaMKII (Cell Signaling Technology, Cat# 12716); rabbit anti-Flag (Proteintech, Cat# 20543-1-AP); rabbit anti-GAD1 (Proteintech, Cat# 10408-1-AP); mouse anti-Gephyrin (Synaptic Systems, Cat# 147 011); rabbit anti-Gephyrin (Proteintech, Cat# 12681-1-AP); mouse anti-GFAP (Cell Signaling Technology, Cat# 3670); mouse anti-GFP (Abmart, Cat# M20004); rabbit anti-Iba1 (Cell Signaling Technology, Cat# 17198); rabbit anti-MDGA1 (Biorbyt, Cat# orb773819); rabbit anti-MDGA2 (Abcam, Cat# ab121164, for immunostaining); rabbit anti-MDGA2 (Biorbyt, Cat# orb540377, for immunoblotting); rabbit anti-mTOR (Cell Signaling Technology, Cat# 2983); rabbit anti-pmTOR (Cell Signaling Technology, Cat# 5536); mouse anti-NeuN (Cell Signaling Technology, Cat# 94403); rabbit anti-NLGN1 (Thermo Fisher Scientific, Cat# PA5-78648); mouse anti-phosphotyrosine (Millipore, Cat# 16-316); rabbit anti-PSD-93 (Abcam, Cat# ab151721); mouse anti-PSD-95 (Synaptic Systems, Cat# 124 011); rabbit anti-PSD-95 (Cell Signaling Technology, Cat# 3450S); mouse anti-puromycin antibody (Millipore, Cat# MABE343); rabbit anti-S6 (Cell Signaling Technology, Cat# 2217); rabbit anti-pS6 (Cell Signaling Technology, Cat# 2211); mouse anti-Synaptophysin (Sigma-Aldrich, Cat# S5768); rabbit anti-TrkB (Cell Signaling Technology, Cat# 4603); rabbit anti-pTrkB (Cell Signaling Technology, Cat# 4619); rabbit anti-GluA1 (Cell Signaling Technology, Cat# 13185); rabbit anti-GluN1 (Cell Signaling Technology, Cat# 5704); mouse anti-vGAT (Santa Cruz Biotechnology, Cat# sc-393373); and mouse anti-vGluT1 (Millipore, Cat# MAB5502). .. The secondary antibodies used include: Goat anti-Mouse IgG (H + L), HRP (ThermoFisher, Cat# 31430); Goat anti-Rabbit IgG (H + L), HRP (ThermoFisher, Cat# 31460); Alexa Fluor 635 Goat anti-Mouse IgG (H + L) (ThermoFisher, Cat# A-31575); Alexa Fluor 488 Goat anti-Rabbit IgG (H + L) (ThermoFisher, Cat#A-11008); and Alexa Fluor 594 Goat anti-Rat IgG (H + L) (ThermoFisher, Cat# A-11007).



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    Image Search Results


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    Journal: Biomedicines

    Article Title: Effects of Continuous Theta Burst Stimulation on Behavior and NMDA Receptor Subunits in the Trimethyltin-Induced Alzheimer’s-like Disease Model

    doi: 10.3390/biomedicines14020391

    Figure Lengend Snippet: Representative immunoblots and quantitative data of Western blot analysis of target proteins expression in hippocampal P2 fraction: ( A ) vGlut1, ( B ) NR1, ( C ) NR2A, ( D ) NR2B. Data were statistically analyzed by one-way ANOVA, followed by Tukey’s post hoc test, and expressed as a percentage of the mean values of the CONT group ± SEM (dots in the graphs represent values of individual animals). The control group is shown in the representative blot images above the graphs but was not included as a separate bar in the graphs to maintain clarity in the main comparisons. Symbols indicate significant differences compared to control: ** p < 0.01, *** p < 0.001, **** p < 0.0001, or Sham cTB. S group: ### p < 0.001, #### p < 0.0001, number of animals per group: 4–6. The can be downloaded at .

    Article Snippet: NR1 , Cell Signaling Technology, Danvers, MA, USA, #5704 RRID: AB_1904067 , rabbit monoclonal; 1:1000.

    Techniques: Western Blot, Expressing, Control